Coding

Part:BBa_K1809000:Design

Designed by: Edward Dring   Group: iGEM15_WPI-Worcester   (2015-07-22)


GaAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 393
    Illegal AgeI site found at 267
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.

Source

Taken from the yeast Glaciozyma antarctica.

References

Pubmed ID: 23132550